Year: 2018 | Month: April | Volume 8 | Issue 1
Effect of Vitamin E on the Quality of Frozen Ram Semen
Abstract:
The objective of the study was to investigate the effect of different concentrations of vitamin E on the quality of frozen-thawed Chhotanagpuri ram semen. A total of 36 ejaculates were collected from six healthy Chhotanagpuri rams with the help of a standard artificial vagina using a restrained ewe as a mount. Two false mounts were allowed before semen was collected. Each semen sample was diluted in Tris extender and divided into three fractions for supplementation of vitamin E at the concentration of 1mM, 2mM and no antioxidant. All the samples immediately after equilibration and 24 hours after freezing and thawing were evaluated for various microscopic parameters viz. sperm motility, live sperm percentage, plasma membrane integrity percentage, acrosomal integrity percentage, DNA integrity and lipid peroxidation assay was evaluated only in freeze thawed semen. Both the doses of vitamin E significantly (PË‚0.05) inhibited lipid peroxidation as indicated by malondialdehyde (MDA) production and improved significantly (PË‚0.05) the values of different seminal characters such as sperm motility, live sperm, plasma membrane integrity, acrosomal integrity and DNA integrity as compared to control Tris dilutor. However, 2 mM vitamin E was most effective as compared to 1mM vitamin E or control Tris dilutor. The percentage of sperm motility, live sperm, plasma membrane, acrosomal and DNA integrity in Tris dilutor containing vitamin E 2mM were found to be 77.83±0.23, 85.86±0.19,¬ 73.03±0.11, 73.81±0.16 and 85.78±0.19, and 56.81±0.41, 65.19±0.18, 54.36±0.17, 55.53±0.27 and 77.97±0.18 after equilibration and freezing, respectively. From the results of the present study, it could be concluded that the addition of vitamin E to Tris freezing medium could reverse the free radical- mediated oxidative damage and improve the quality of frozen thawed Chhotanagpuri ram semen.
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